Reconstitution Guide (with Dilution Examples)

This guide explains how lyophilized peptides are typically reconstituted using bacteriostatic water (BAC water). It is intended for informational and laboratory handling purposes only. Always follow strict sterile technique and seek professional guidance where appropriate.

What is Reconstitution?

Reconstitution is the process of adding a sterile diluent (commonly bacteriostatic water) to a freeze-dried peptide vial in order to form a liquid solution suitable for handling and further preparation.

What You Need

Lyophilized peptide vial (10mg / 20mg / 30mg / 40mg)
Bacteriostatic water (BAC water)
Alcohol swabs (70% isopropyl alcohol)
Sterile syringe and needles
Clean, sterile workspace

General Principle

Once BAC water is added, the concentration depends on the amount of liquid used.

Formula:

Concentration (mg/mL) = Total peptide (mg) ÷ Total BAC water added (mL)

Example Dilution Chart (BAC Water Reconstitution)

10mg Vial

BAC Water Added	Final Concentration
1 mL	10 mg/mL
2 mL	5 mg/mL
3 mL	3.33 mg/mL
4 mL	2.5 mg/mL

20mg Vial

BAC Water Added	Final Concentration
1 mL	20 mg/mL
2 mL	10 mg/mL
3 mL	6.67 mg/mL
4 mL	5 mg/mL

30mg Vial

BAC Water Added	Final Concentration
1 mL	30 mg/mL
2 mL	15 mg/mL
3 mL	10 mg/mL
4 mL	7.5 mg/mL

40mg Vial

BAC Water Added	Final Concentration
1 mL	40 mg/mL
2 mL	20 mg/mL
3 mL	13.33 mg/mL
4 mL	10 mg/mL

Important Handling Notes

Always inject BAC water slowly down the side of the vial
Do not shake vigorously — gently swirl instead
Allow powder to fully dissolve before use
Use sterile equipment at all times
Discard if solution appears cloudy or contaminated

Storage After Reconstitution

Store at 2–8°C (refrigerated)
Do not freeze
Keep vial sealed and protected from light
Use sterile technique each time the vial is accessed

Safety Notice

This content is for informational purposes only and does not constitute medical advice. Handling of peptides should be carried out responsibly and in accordance with applicable laws and professional guidance.

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